Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso

Benjamin Ouédraogo; Alphonsine Ramdé Tiendrébéogo; Jules Yoda; Felix Kini

doi:doi:10.11648/j.sjc.20241205.12

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Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso

Benjamin Ouédraogo

, Alphonsine Ramdé Tiendrébéogo

, Jules Yoda^*

, Felix Kini

Published in Science Journal of Chemistry (Volume 12, Issue 5)

Received: 2 August 2024 Accepted: 2 September 2024 Published: 20 September 2024

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Abstract

The aim of this study is to analyze the phytochemical composition and radical scavenging activity of three leafy vegetables from Burkina Faso: Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius. Samples of these plants were collected in Zitenga region, dried, powdered and extracted by decoction using water and a hydroethanolic solvent. Phytochemical screening was carried out by thin layer chromatography. Bioactive compounds, notably flavonoids and phenolic compounds, were measured respectively by the aluminum trichloride (AlCl₃) method and the Folin-Ciocalteu method. The radical scavenging activity of the extracts was also investigated using the DPPH method. Phytochemical analyzes revealed the presence of bioactive compounds such as flavonoids, tannins and saponosides in the three species studied. The highest total phenolic and total flavonoid contents were obtained respectively with the hydroethanolic extract of Cleome gynandra (223.54 ± 0.18 mg TEA/g DM) and the aqueous extract of Cleome gynandra (69.09 ± 0.26 mg QE/g DM). The best antioxidant activity was obtained with the hydroethanolic extract of Corchorus olitorius (IC₅₀: 31.93 ± 1.95 µg /mL). The results showed that the extracts of Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius all exhibited radical scavenging activity, indicating their potential to reduce oxidative stress. These results suggest that Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius are rich sources of health-promoting phytochemicals. These food plants from Burkina Faso could therefore be transformed into nutraceuticals to promote health and prevent diseases linked to oxidative stress.

Published in	Science Journal of Chemistry (Volume 12, Issue 5)
DOI	10.11648/j.sjc.20241205.12
Page(s)	94-101
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Phytochemical Composition, Cleome Gynandra, Hibiscus Sabdariffa, Corchorus Olitorius, Radical Scavenging

References

[1]	F. L. Lukoki, D. N. Musika, B. Z. Gbolo, A. B. Kikufi, and H. N. Lukoki, “Étude phyto-chimique et activité glucophage in vitro de Laportea aestuans (L.) Chew. (Urticaceae),” vol. 11, no. 1, pp. 49–53, 2023.
[2]	A. Ramdé-Tiendrébéogo, M. Koala, “Comparative study of phytochemical profile and antioxidant activity of sahelian plants used in the treatment of childhood diseases in northern Burkina Faso: case of Petroselinum crispum (Mill.) Fuss, Ocimum gratissimum L and Acacia senegal (L.) Willd’,” Res. J. Chem. Environ. Sci., vol. 4, pp. 9–17, 2019.
[3]	N. T. R. Meda, M. J. Bangou, S. Bakasso, and O. G. Nacoulma, “Antioxidant activity of phenolic and flavonoid fractions of Cleome gynandra and Maerua angolensis of Burkina Faso,” J. Appl. Pharm. Sci., vol. 3, no. 02, pp. 36–42, 2013. https://doi.org/10.7324/JAPS.2013.30207
[4]	J. Bruneton, “Pharmacognosie-Phytochimie, plantes médicinales,” Tec Doc-Éditions médicales Int., 4e éd., revue et augmentée, Paris, p. 1288, 2009.
[5]	S. A. Mohd Ali, C. R. Che Mohd, and J. Latip, “Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times,” Sains Malaysiana, vol. 48, no. 7, pp. 1417–1424, 2019. https://doi.org/10.17576/jsm-2019-4807-10
[6]	M. Cisse, M. Dornier, M. Sakho, A. Ndiaye, M. Reynes, and O. Sock, “Le bissap (Hibiscus sabdariffa L.): Composition et principales utilisations,” EDP Sci., vol. 64, no. 3, pp. 179–193, 2009. https://doi.org/10.1051/fruits/2009013
[7]	B. D. and D. B. Buyile Ncube, “Effect of Common Cooking and Drying Methods on Phytochemical and Antioxidant Properties of Corchorus olitorius Identified Using Liquid Chromatography-Mass,” Molecules, vol. 27, 9052, pp. 1–15, 2022. https://doi.org/10.3390/molecules27249052
[8]	M. Osaronowen, E. Udo, and S. Oricha, “Anti diabetic Activity Of Ethanolic Seed Extract Of Corchorus olitorius,” Int. J. Sci. Basic Appl. Res., vol. 12, no. 1, pp. 8–22, 2013.
[9]	AOAC, “Official methods of Analysis of AOAC International.,” Int. A, éditeur. Washington, DC, USA, vol. 16th éd, 1999.
[10]	Wagner H. & Bladt, “Plant drug analysis,” a thin layer Chromatogr. 2 nd Ed. Berlin, p. 384 P, 1996.
[11]	V. L. Singleton, “Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent.,” Methods Enzymol., vol. 229, pp. 152–78, 1999.
[12]	A. Kumaran, R. J. Karunakaran, “In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT -,” J Food Sci. Technol., vol. 2, no. 40, pp. 344–52, 2005. https://doi.org/10.1016/j.lwt.2005.09.011
[13]	El-Sayed Saleh Abdel-Hameed, “Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples,” Food Chem., vol. 4, no. 114, pp. 271-277., 2009. https://doi.org/10.1016/j.foodchem.2008.11.005
[14]	K. S. Kim, S. Lee, Y. S. Lee, S. H. Jung, Y. Park, K. H. Shin, B-K. Kim., “Anti-oxidant activities of the extracts from the herbs of Artemisia apiacea,” J. Ethnopharmacol., vol. 85, no. 2, pp. 69–72, 2003. https://doi.org/10.1016/S0378-8741(02)00338-0
[15]	J. Yoda, J. C. Ouedraogo, S. Ouedraogo, M. Traore-Coulibaly, F. Kini, M. Lompo, S. Ouedraogo, “ Standardisation Process of Saye, a Traditional Polyherbal Formulation Remedy for Malaria: Physico-chemical Analysis and Phytochemical Investigation,” Science Journal of Analytical Chemistry, vol.8, no. 1, pp. 33-40, 2020. https://doi.org/10.11648/j.sjac.20200801.16
[16]	J. Y. Chabrier, “Plantes médicinales et formes d’utilisation en Phytothérapie,” Sciences pharmaceutiques. 2010.
[17]	N. M. Souhila, K. Mustapha, “Etude de l ’ extraction des composés phénoliques de différentes parties de la fleur d ’ artichaut (Cynara scolymus L.),” Nat. Technol., vol. 3, no. 1, pp. 5–9, 2013.
[18]	S. Kagale, T. Marinuithu, B. Thayumanavan, R. Nandakumar, R. Samiyappan, “Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. Oryzae.,” Physiol. Mol. Plant Pathol., vol. 65, no.2, pp. 91-100, 2004. https://doi.org/10.1016/j.pmpp.2004.11.008
[19]	B. Bamba, C. K. D. Benie, A. Ouattara, D. N. Doukourou, R. K. Kamou, and K. Ouattara, “Total phenol content, antioxidant activities of macerated and decocted leaves of Uvaria chamae P. Beauv. (Annonaceae),” Int. J. Biol. Chem. Sci., vol. 15, no. 1, pp. 54–67, 2021. https://doi.org/10.4314/ijbcs.v15i1.6
[20]	K. S. Evenamede, K. Kpegba, O. Simalou, P. Boyode, and A. Agbonon, “ Etude comparative des activités antioxydantes d’extraits éthanoliques de feuilles, d’écorces et de racines de Cassia sieberiana,” Int. J. Biol. Chem. Sci., vol. 11, no. 6, pp. 2924–2935, 2017. https://doi.org/10.4314/ijbcs.v11i6.29
[21]	K. Fadili, S. Amalich, S. K. N’dedianhoua, M. Bouachrine, M. Mahjoubi, F. E. Hilali, and T. Zair, “Teneurs en polyphénols et évaluation de l’activité antioxydante des extraits de deux espèces du Haut Atlas du Maroc: Rosmarinus Officinalis et Thymus Satureioides [Polyphenols content and antioxidant activity of two species from Moroccan High Atlas: Rosm,” Int. J. Innov. Sci. Res., vol. 17, no. 1, pp. 24–33, 2015.
[22]	R. M. Habellah, S. Karoune, M. S. A. Kechebar and H. Bounab, “Etude des composés phénoliques et des activités antioxydantes de l’Acacia ehrenbergiana de la région de Tindouf,” J. Algérien des Régions Arides, vol. 13, pp. 27–34, 2016.
[23]	S. P. Sandhar HK., Kumar B., Prasher S., Tiwari P., Salhan M., “A review of phytochemistry and pharmacology of flavonoids,” Int. Pharm. Sci., vol. 1, pp. 25–41, 2011. https://ci.nii.ac.jp/naid/10007369526
[24]	M. J. Allcarz, M. J. Jiménez, “Flavonoids as anti-inflammatory agents,” Fitoterapia, vol. 59, no.1, pp. 25–38, 1988.
[25]	V. Cody, E. Middleton., J. B. Harborne, ‘Plant flavonoids in biology and medicine: biochemical, pharmacological, and structure- activity relationships’, proceedings of a symposium held in Buffalo,” Prog. Clin. Biol. Res., 1986.
[26]	A. F. Jospin, A. A. Boya, O. P. Abiodoun, F. Assougba and F. Houndonougbo, “Valeurs nutritives, propriétés antioxydantes et cytotoxicité des cossettes de Dioscorea dumetorum pour la valorisation en alimentation animale et humaine au Bénin,”Tropicultura, vol. 40, no.1, pp. 1–16, 2022. https://doi.org/10.25518/2295-8010.1963

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APA Style

Ouédraogo, B., Tiendrébéogo, A. R., Yoda, J., Kini, F. (2024). Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso. Science Journal of Chemistry, 12(5), 94-101. https://doi.org/10.11648/j.sjc.20241205.12

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ACS Style

Ouédraogo, B.; Tiendrébéogo, A. R.; Yoda, J.; Kini, F. Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso. Sci. J. Chem. 2024, 12(5), 94-101. doi: 10.11648/j.sjc.20241205.12

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AMA Style

Ouédraogo B, Tiendrébéogo AR, Yoda J, Kini F. Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso. Sci J Chem. 2024;12(5):94-101. doi: 10.11648/j.sjc.20241205.12

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@article{10.11648/j.sjc.20241205.12,
  author = {Benjamin Ouédraogo and Alphonsine Ramdé Tiendrébéogo and Jules Yoda and Felix Kini},
  title = {Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso
},
  journal = {Science Journal of Chemistry},
  volume = {12},
  number = {5},
  pages = {94-101},
  doi = {10.11648/j.sjc.20241205.12},
  url = {https://doi.org/10.11648/j.sjc.20241205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20241205.12},
  abstract = {The aim of this study is to analyze the phytochemical composition and radical scavenging activity of three leafy vegetables from Burkina Faso: Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius. Samples of these plants were collected in Zitenga region, dried, powdered and extracted by decoction using water and a hydroethanolic solvent. Phytochemical screening was carried out by thin layer chromatography. Bioactive compounds, notably flavonoids and phenolic compounds, were measured respectively by the aluminum trichloride (AlCl3) method and the Folin-Ciocalteu method. The radical scavenging activity of the extracts was also investigated using the DPPH method. Phytochemical analyzes revealed the presence of bioactive compounds such as flavonoids, tannins and saponosides in the three species studied. The highest total phenolic and total flavonoid contents were obtained respectively with the hydroethanolic extract of Cleome gynandra (223.54 ± 0.18 mg TEA/g DM) and the aqueous extract of Cleome gynandra (69.09 ± 0.26 mg QE/g DM). The best antioxidant activity was obtained with the hydroethanolic extract of Corchorus olitorius (IC50: 31.93 ± 1.95 µg /mL). The results showed that the extracts of Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius all exhibited radical scavenging activity, indicating their potential to reduce oxidative stress. These results suggest that Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius are rich sources of health-promoting phytochemicals. These food plants from Burkina Faso could therefore be transformed into nutraceuticals to promote health and prevent diseases linked to oxidative stress.
},
 year = {2024}
}

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TY  - JOUR
T1  - Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso

AU  - Benjamin Ouédraogo
AU  - Alphonsine Ramdé Tiendrébéogo
AU  - Jules Yoda
AU  - Felix Kini
Y1  - 2024/09/20
PY  - 2024
N1  - https://doi.org/10.11648/j.sjc.20241205.12
DO  - 10.11648/j.sjc.20241205.12
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 94
EP  - 101
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20241205.12
AB  - The aim of this study is to analyze the phytochemical composition and radical scavenging activity of three leafy vegetables from Burkina Faso: Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius. Samples of these plants were collected in Zitenga region, dried, powdered and extracted by decoction using water and a hydroethanolic solvent. Phytochemical screening was carried out by thin layer chromatography. Bioactive compounds, notably flavonoids and phenolic compounds, were measured respectively by the aluminum trichloride (AlCl3) method and the Folin-Ciocalteu method. The radical scavenging activity of the extracts was also investigated using the DPPH method. Phytochemical analyzes revealed the presence of bioactive compounds such as flavonoids, tannins and saponosides in the three species studied. The highest total phenolic and total flavonoid contents were obtained respectively with the hydroethanolic extract of Cleome gynandra (223.54 ± 0.18 mg TEA/g DM) and the aqueous extract of Cleome gynandra (69.09 ± 0.26 mg QE/g DM). The best antioxidant activity was obtained with the hydroethanolic extract of Corchorus olitorius (IC50: 31.93 ± 1.95 µg /mL). The results showed that the extracts of Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius all exhibited radical scavenging activity, indicating their potential to reduce oxidative stress. These results suggest that Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius are rich sources of health-promoting phytochemicals. These food plants from Burkina Faso could therefore be transformed into nutraceuticals to promote health and prevent diseases linked to oxidative stress.

VL  - 12
IS  - 5
ER  -

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Author Information

Benjamin Ouédraogo

Research and Development Laboratory (LRD), University Ledea Bernard Ouedraogo (ULBO), Ouahigouya, Burkina Faso; International Research Laboratory IRL 3189 Environment, Health, Societies (CNRST, USTTB, UCAD, UGB, CNRS), Ouagadougou, Burkina Faso

Contact Email

http://orcid.org/0009-0008-6748-9812
Alphonsine Ramdé Tiendrébéogo

Department of Traditional Medicine, Pharmacopoeia – Pharmacy (MEPHATRA-PH), Health Sciences Research Institute (IRSS /CNRST), Ouagadougou, Burkina Faso; International Research Laboratory IRL 3189 Environment, Health, Societies (CNRST, USTTB, UCAD, UGB, CNRS), Ouagadougou, Burkina Faso

Contact Email

http://orcid.org/0009-0009-0074-0715
Jules Yoda

Department of Traditional Medicine, Pharmacopoeia – Pharmacy (MEPHATRA-PH), Health Sciences Research Institute (IRSS /CNRST), Ouagadougou, Burkina Faso

Contact Email

http://orcid.org/0000-0001-5496-0814
Felix Kini

Department of Traditional Medicine, Pharmacopoeia – Pharmacy (MEPHATRA-PH), Health Sciences Research Institute (IRSS /CNRST), Ouagadougou, Burkina Faso

Contact Email

http://orcid.org/0009-0002-8752-9902

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Ouédraogo, B., Tiendrébéogo, A. R., Yoda, J., Kini, F. (2024). Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso. Science Journal of Chemistry, 12(5), 94-101. https://doi.org/10.11648/j.sjc.20241205.12

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ACS Style

Ouédraogo, B.; Tiendrébéogo, A. R.; Yoda, J.; Kini, F. Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso. Sci. J. Chem. 2024, 12(5), 94-101. doi: 10.11648/j.sjc.20241205.12

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AMA Style

Ouédraogo B, Tiendrébéogo AR, Yoda J, Kini F. Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso. Sci J Chem. 2024;12(5):94-101. doi: 10.11648/j.sjc.20241205.12

Copy | Download

@article{10.11648/j.sjc.20241205.12,
  author = {Benjamin Ouédraogo and Alphonsine Ramdé Tiendrébéogo and Jules Yoda and Felix Kini},
  title = {Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso
},
  journal = {Science Journal of Chemistry},
  volume = {12},
  number = {5},
  pages = {94-101},
  doi = {10.11648/j.sjc.20241205.12},
  url = {https://doi.org/10.11648/j.sjc.20241205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20241205.12},
  abstract = {The aim of this study is to analyze the phytochemical composition and radical scavenging activity of three leafy vegetables from Burkina Faso: Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius. Samples of these plants were collected in Zitenga region, dried, powdered and extracted by decoction using water and a hydroethanolic solvent. Phytochemical screening was carried out by thin layer chromatography. Bioactive compounds, notably flavonoids and phenolic compounds, were measured respectively by the aluminum trichloride (AlCl3) method and the Folin-Ciocalteu method. The radical scavenging activity of the extracts was also investigated using the DPPH method. Phytochemical analyzes revealed the presence of bioactive compounds such as flavonoids, tannins and saponosides in the three species studied. The highest total phenolic and total flavonoid contents were obtained respectively with the hydroethanolic extract of Cleome gynandra (223.54 ± 0.18 mg TEA/g DM) and the aqueous extract of Cleome gynandra (69.09 ± 0.26 mg QE/g DM). The best antioxidant activity was obtained with the hydroethanolic extract of Corchorus olitorius (IC50: 31.93 ± 1.95 µg /mL). The results showed that the extracts of Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius all exhibited radical scavenging activity, indicating their potential to reduce oxidative stress. These results suggest that Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius are rich sources of health-promoting phytochemicals. These food plants from Burkina Faso could therefore be transformed into nutraceuticals to promote health and prevent diseases linked to oxidative stress.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Phytochemical Study and Radical Scavenging Activity of Three Leafy Vegetables Grown in Zitenga Region of Burkina Faso

AU  - Benjamin Ouédraogo
AU  - Alphonsine Ramdé Tiendrébéogo
AU  - Jules Yoda
AU  - Felix Kini
Y1  - 2024/09/20
PY  - 2024
N1  - https://doi.org/10.11648/j.sjc.20241205.12
DO  - 10.11648/j.sjc.20241205.12
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 94
EP  - 101
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20241205.12
AB  - The aim of this study is to analyze the phytochemical composition and radical scavenging activity of three leafy vegetables from Burkina Faso: Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius. Samples of these plants were collected in Zitenga region, dried, powdered and extracted by decoction using water and a hydroethanolic solvent. Phytochemical screening was carried out by thin layer chromatography. Bioactive compounds, notably flavonoids and phenolic compounds, were measured respectively by the aluminum trichloride (AlCl3) method and the Folin-Ciocalteu method. The radical scavenging activity of the extracts was also investigated using the DPPH method. Phytochemical analyzes revealed the presence of bioactive compounds such as flavonoids, tannins and saponosides in the three species studied. The highest total phenolic and total flavonoid contents were obtained respectively with the hydroethanolic extract of Cleome gynandra (223.54 ± 0.18 mg TEA/g DM) and the aqueous extract of Cleome gynandra (69.09 ± 0.26 mg QE/g DM). The best antioxidant activity was obtained with the hydroethanolic extract of Corchorus olitorius (IC50: 31.93 ± 1.95 µg /mL). The results showed that the extracts of Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius all exhibited radical scavenging activity, indicating their potential to reduce oxidative stress. These results suggest that Cleome gynandra, Hibiscus sabdariffa and Corchorus olitorius are rich sources of health-promoting phytochemicals. These food plants from Burkina Faso could therefore be transformed into nutraceuticals to promote health and prevent diseases linked to oxidative stress.

VL  - 12
IS  - 5
ER  -

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